ligation enhancer (New England Biolabs)
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New England Biolabs
ligation enhancer
Ligation Enhancer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 3123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ligation+enhancer/NEBNext+Multi+Olig+IL+96+Dual+index+prim/bio_rxiv__64898__2026__03__26__714241-204-27-29
Average 97 stars, based on 3123 article reviews
Ligation Enhancer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 3123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ligation+enhancer/NEBNext+Multi+Olig+IL+96+Dual+index+prim/bio_rxiv__64898__2026__03__26__714241-204-27-29
Average 97 stars, based on 3123 article reviews
ligation enhancer - by Bioz Stars,
2026-10
97/100 stars
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Ligation:Article Title: 3D genome organization shapes DNA damage susceptibility to platinum-based drugs Article Snippet: The fragmented genomic DNA (∼2 μg) was used for end repair, 5′ phosphorylation, and dA-Tailing using 3 μl of End Prep Enzyme Mix and 7 μl of End Prep Reaction Buffer (NEB #E7546 or as included in NEB #E7645), at 20°C for 30 min and then 65°C for 30 min, before cooling down to room temperature. .. Following this, annealed AD1 adapter (500 pmol) was ligated to End Prepped DNA with 30 μl of Ligation Master Mix and 1 μl of Article Title: An i-motif-regulated enhancer, eRNA and adjacent lncRNA affect Lhb expression through distinct mechanisms in a sex-specific context. Article Snippet: The fragmented DNA was end-repaired and dA-tailed (NEBNext® UltraTM II End Repair/dA-Tailing Module; NEB #E7546), and 5′-end dephosphorylated with calf intestinal alkaline phosphatase (New England Biolabs, M0525) for 1 h at 37 °C. .. The DNA was purified with 1x Ampure XP beads, eluted in 60 μl of water, and then ligated with the Illumina adapter using the NEBNext Ultra II Ligation Module, with 2.5 μl NEBNext Illumina adapter (final 0.4 μM), 30 μl NEBNext Ultra II Ligation Master Mix, and 1 μl of Article Title: An i-motif-regulated enhancer, eRNA and adjacent lncRNA affect Lhb expression through distinct mechanisms in a sex-specific context Article Snippet: The fragmented DNA was end-repaired and dA-tailed (NEBNext ® UltraTM II End Repair/dA-Tailing Module; NEB #E7546), and 5′-end dephosphorylated with calf intestinal alkaline phosphatase (New England Biolabs, M0525) for 1 h at 37 °C. .. The DNA was purified with 1x Ampure XP beads, eluted in 60 μl of water, and then ligated with the Illumina adapter using the NEBNext Ultra II Ligation Module, with 2.5 μl NEBNext Illumina adapter (final 0.4 μM), 30 μl NEBNext Ultra II Ligation Master Mix, and 1 μl of Article Title: Identification of Parkinson’s disease-associated regulatory variants in human dopaminergic neurons reveals modulators of SCARB2 and BAG3 expression Article Snippet: The reaction was carried on in a Analytik Jena Biometra thermocycler with the heated lid ON and the following program: 30 minutes at 20°C, 30 minutes at 65°C and hold at 4°C. .. Addition of indexes was performed by adding 15 μl of Blunt/TA Ligase master mix (NEB E6440S), 2.5 μl of NEBNext Adaptor (NEB E6440S) and 1.0 μl of Article Title: Loop Extrusion Accelerates Long-Range Enhancer-Promoter Searches in Living Embryos Article Snippet: Biotin labeled DNA were then extracted by binding to Streptavidin beads (Dynabeads C1-Invitrogen Cat no. 65-001). .. The DNA ends were processed for adapter ligation by treatment with 3.5 μl of End Prep Reaction buffer and 1.5 μl End Prep enzyme mix (NEB-E7645S) in a 30 μl reaction incubate at 20 °C for 30 min and then at 65 °C for 30 min. Adapter ligation was then performed by the addition of 0.5 μl Illumina adapter, 15 μl of Ultra II Ligation Master Mix and 0.5 μl Article Title: Extensive transmission and variation in a functional receptor for praziquantel resistance in endemic Schistosoma mansoni Article Snippet: .. Adaptor ligated libraries were prepared by adding 30 μL of ligation mix, 1 μL Article Title: Methyl-CODEC enables simultaneous methylation and duplex sequencing Article Snippet: Subsequently, DNA purification was performed using 1.8X AMPure XP beads according to the manufacturer’s protocol (Beckman Coulter), followed by elution in 60 μl of low TE buffer. .. Adaptor ligation was performed by adding 30 μl of Ligation Master Mix, 1 μl of Article Title: Splint nucleic acid molecule for cyclizing single-stranded nucleic acid molecule and use thereof Article Snippet: .. 9) Cyclization A cyclization reaction mixture containing 12 μl ligation buffer, 1.2 μl Purification:Article Title: An i-motif-regulated enhancer, eRNA and adjacent lncRNA affect Lhb expression through distinct mechanisms in a sex-specific context. Article Snippet: The fragmented DNA was end-repaired and dA-tailed (NEBNext® UltraTM II End Repair/dA-Tailing Module; NEB #E7546), and 5′-end dephosphorylated with calf intestinal alkaline phosphatase (New England Biolabs, M0525) for 1 h at 37 °C. .. The DNA was purified with 1x Ampure XP beads, eluted in 60 μl of water, and then ligated with the Illumina adapter using the NEBNext Ultra II Ligation Module, with 2.5 μl NEBNext Illumina adapter (final 0.4 μM), 30 μl NEBNext Ultra II Ligation Master Mix, and 1 μl of Article Title: An i-motif-regulated enhancer, eRNA and adjacent lncRNA affect Lhb expression through distinct mechanisms in a sex-specific context Article Snippet: The fragmented DNA was end-repaired and dA-tailed (NEBNext ® UltraTM II End Repair/dA-Tailing Module; NEB #E7546), and 5′-end dephosphorylated with calf intestinal alkaline phosphatase (New England Biolabs, M0525) for 1 h at 37 °C. .. The DNA was purified with 1x Ampure XP beads, eluted in 60 μl of water, and then ligated with the Illumina adapter using the NEBNext Ultra II Ligation Module, with 2.5 μl NEBNext Illumina adapter (final 0.4 μM), 30 μl NEBNext Ultra II Ligation Master Mix, and 1 μl of Article Title: Splint nucleic acid molecule for cyclizing single-stranded nucleic acid molecule and use thereof Article Snippet: .. 9) Cyclization A cyclization reaction mixture containing 12 μl ligation buffer, 1.2 μl Incubation:Article Title: Identification of Parkinson’s disease-associated regulatory variants in human dopaminergic neurons reveals modulators of SCARB2 and BAG3 expression Article Snippet: The reaction was carried on in a Analytik Jena Biometra thermocycler with the heated lid ON and the following program: 30 minutes at 20°C, 30 minutes at 65°C and hold at 4°C. .. Addition of indexes was performed by adding 15 μl of Blunt/TA Ligase master mix (NEB E6440S), 2.5 μl of NEBNext Adaptor (NEB E6440S) and 1.0 μl of Article Title: Loop Extrusion Accelerates Long-Range Enhancer-Promoter Searches in Living Embryos Article Snippet: Biotin labeled DNA were then extracted by binding to Streptavidin beads (Dynabeads C1-Invitrogen Cat no. 65-001). .. The DNA ends were processed for adapter ligation by treatment with 3.5 μl of End Prep Reaction buffer and 1.5 μl End Prep enzyme mix (NEB-E7645S) in a 30 μl reaction incubate at 20 °C for 30 min and then at 65 °C for 30 min. Adapter ligation was then performed by the addition of 0.5 μl Illumina adapter, 15 μl of Ultra II Ligation Master Mix and 0.5 μl Article Title: Extensive transmission and variation in a functional receptor for praziquantel resistance in endemic Schistosoma mansoni Article Snippet: .. Adaptor ligated libraries were prepared by adding 30 μL of ligation mix, 1 μL Adapter Ligation:Article Title: Loop Extrusion Accelerates Long-Range Enhancer-Promoter Searches in Living Embryos Article Snippet: Biotin labeled DNA were then extracted by binding to Streptavidin beads (Dynabeads C1-Invitrogen Cat no. 65-001). .. The DNA ends were processed for adapter ligation by treatment with 3.5 μl of End Prep Reaction buffer and 1.5 μl End Prep enzyme mix (NEB-E7645S) in a 30 μl reaction incubate at 20 °C for 30 min and then at 65 °C for 30 min. Adapter ligation was then performed by the addition of 0.5 μl Illumina adapter, 15 μl of Ultra II Ligation Master Mix and 0.5 μl Polymerase Chain Reaction:Article Title: Splint nucleic acid molecule for cyclizing single-stranded nucleic acid molecule and use thereof Article Snippet: .. 9) Cyclization A cyclization reaction mixture containing 12 μl ligation buffer, 1.2 μl |